酶免分析法检测甲状腺双抗体在临床中的应用
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2010年11月15日
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[摘要] 目的：探讨抗甲状腺球蛋白抗体(Anti-Tg)和抗甲状腺过氧化物酶抗体(Anti-TPO)检测在临床上较高阳性率。方法：采用微粒子酶免分析法(MEIA)分析医院就诊疑似甲状腺疾病的患者225例，并与健康体检人群42例检测Anti-Tg和Anti-TPO检测阳性率进行对照。结果：医院就诊疑似甲状腺疾病的患者阳性率(Anti-Tg为40.9%、Anti-TPO为31.6%)明显高于健康体检人群阳性率(Anti-Tg为16.7%、Anti-TPO为14.3%)，两组比较差异有统计学意义。结论：通过MEIA检测血清中Anti-Tg 和Anti-TPO水平，与甲状腺疾病有密切关系，可作为甲状腺疾病的诊断指标。MEIA对Anti-Tg 和Anti-TPO进行定量测定，其检测速度快、灵敏度高、重复性好，自动化程度高，具有良好的稳定性，检测效率高，能及时为临床上诊断、治疗甲状腺疾病提供实验依据，值得推广。
[关键词] 微粒子酶免分析法；抗甲状腺球蛋白抗体；抗甲状腺过氧化物酶抗体
[中图分类号] R-331[文献标识码] B [文章编号] 1674-4721(2010)11(b)-066-02
ELISA double antibody assay of thyroid clinical application
CUI Mingshu1, ZHU Chengtun2
(1.The Hospital of Yanji City, Jilin Province, Yanji 133000, China; 2.Yanbian Blood Staion of Jilin Province, Yanji 133000, China)
[Abstract] Objective: To explore the high positive rate of thyroglobulin antibody (Anti-Tg) and anti-thyroid peroxidase antibody(Anti-TPO) detection in clinic. Methods: The hospital examined 225 patients suspected of thyroid disease, using micro particle enzyme immunoassay (MEIA), and compared it to the positive rate of 42 clients of health examination by Anti-Tg and Anti-TPO detection. Results: The positive rate of the patients suspected of thyroid disease(Anti-Tg was 40.9%, Anti-TPO was 31.6%) was apparently higher than the healthy clients (Anti-Tg was 16.7%, Anti-TPO was 14.3%), and two groups of comparisons have obvious statistical differences. Conclusion: Closely relate to the thyroid disease, the Anti-Tg and Anti-TPO level in serum results from MEIA detection, can be applied as a diadynamic criteria. MEIA conducts quantitative determination to Anti-Tg and Anti-TPO, with high detection speed, sensitivity, repeatability, and high degree of automation, credible stability and efficiency. It can be provide timely experimental evidence for clinical diagnosis and treatment of thyroid disease, and is worth to be popularized.
[Key words] Micro particle enzyme immunoassay; Anti-Tg; Anti-TPO
本文对本院就诊疑似甲状腺疾病患者225例进行分析，现报道如下：
1 资料与方法
1.1 一般资料
健康组：自愿无偿献血人群中无异常指标者20例，年龄18～40岁。普查组：延吉市医院健康体检人群中随机抽样，42例，年龄20～50岁。医院就诊患者组：在延吉市医院就诊疑似甲状腺疾病的患者225例，年龄10～80岁，男68例，女157例，朝鲜族132例，汉族93例。
1.2 标本处理
血液标本空腹采集，立即注入试管中，在室温放置待凝结后离心取血清待测。
1.3 方法
1.3.1 检测试剂美国雅培试剂，仪器配套的成套试剂。
1.3.2 监测仪器美国雅培公司生产的AXSYM全自动化学发光分析仪。
1.3.3 检测方法微粒子酶免分析法(MEIA)，按操作说明书进行操作。
2 结果
2.1健康组、普查组和医院就诊患者检测结果
抗甲状腺球蛋白抗体(Anti-Tg)检测值大于34 IU/ml者为阳性，抗甲状腺过氧化物酶抗体(Anti-TPO)检测值大于12 IU/ml者为阳性。健康组、普查组和医院就诊患者检测结果见表1。
2.2 Anti-Tg、Anti-TPO阳性率
医院就诊患者组Anti-Tg阳性率为40.9%，明显高于普查组的16.7%，差异有统计学意义(P<0.01)；医院就诊患者Anti-TPO阳性率为31.6%，明显高于普查组的14.3%，差异有统计学意义(P<0.05)。健康组20例中1例Anti-Tg出现阳性(43 IU/ml)结果，其余均为阴性结果。
3 讨论
本实验中，医院就诊疑似甲状腺疾病的患者阳性率(Anti-Tg为40.9%、Anti-TPO为31.6%)明显高于健康体检人群阳性率(Anti-Tg为16.7%、Anti-TPO为14.3%)，两组比较差异有统计学意义[1]。血清中Anti-Tg 和Anti-TPO水平 ......
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