复方茯苓甘草汤防治慢性常压低氧性肺动脉高压的有效成分筛选实验研究(1)
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摘 要:目的:通过实验来筛选出复方茯苓甘草汤防治慢性肺动脉高压(PAH)的有效成分。方法:采用正交试验法,以平均肺动脉压、右心指数和肺血管形态学的改变为指标,进行整体动物的药效学试验,观察各分离成分对PAH的防治作用。结果:可认为60%液与95%液能使大鼠模型mPAP、RV/(LV+S)、MA(%)、PMA(%)和MA/ LA降低,NMA(%)则升高;而蒸洗液与30%液不能起效。尚不能认为蒸洗液、30%液、60%液、95%液这4种提取液之间的一级交互作用和二级交互作用对大鼠模型上述指标有影响。结论:筛选出60%液、95%液这两个有效部位(群)/有效成分(群)作为复方茯苓甘草汤防治常压缺氧性PAH大鼠模型的主要物质基础。
关键词:复方茯苓甘草汤;肺动脉高压;有效成分
中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)04-0786-03
Screen Assay of the Active Ingredient from Fufang Fuling Gancao DecoctionAgainst Chronic Normobaric Hypoxic Pulmonary Arterial Hypertension
FAN Weiying,WANG Bozhang
(Affiliated Hospital of Guangdong Medical College, Zhanjiang 524023, Guangdong, China)
Abstract:Objective: To identify an active ingredient that could be used in the treatment of chronic pulmonary arterial hypertension (PAH) from the Fufang Fuling Gancao Decoction.Methods:In the studies, the experiment was performed by orthogonal design, the mPAP,RV/ (LV+S) and related indexes of pneumoangiogram morphology were regarded as criterion for evaluation of the every composition against PAH. Results: Ex.60% and Ex.95% could decrease mPAP,RV/(LV+S),MA(%),PMA(%),MA/ LA and increase NMA(%)of rat model, but could not Ex.D.W and Ex.30%.It could not be considered that two-way interactions or second-order interactions among these four compositions had any effect upon all the related indexes of rat model.Conclusion: Ex.60% and Ex.95% could be deemed to be the active ingredients of Fufang Fuling Gancao Decoction against rat model of chronic normobaric hypoxic PAH.
Key words:Fufang Fuling Gancao Decoction; pulmonary arterial hypertension; active ingredient
收稿日期:2009-11-10
基金项目:广东省中医药局重点课题资助项目(3060007)
作者简介:范伟赢(1981-),男,广东罗定人,医师,硕士,研究方向:呼吸系统疾病。
复方茯苓甘草汤化裁于东汉末年医圣张仲景所著《伤寒杂病论》的茯苓甘草汤,结合现代临床实践而成。本文将复方茯苓甘草汤作为一个整体,通过建立慢性常压缺氧性肺动脉高压(Pulmonary Arterial Hypertension, PAH)大鼠模型,分别以肺动脉压、肺血管形态学和右心指数的改变为指标,进行整体动物的药效学试验,观察各分离成分对PAH的防治作用,筛选出该方防治慢性PAH的有效成分。
1 材 料
1.1 药材 茯苓、甘草、桂枝、桃仁、知母等由广东医学院附属医院中药房提供。
1.2 灌胃药液成分的制备 HP20型大孔吸附树脂分离复方茯苓甘草汤全方水煎液(浓度为2g生药/mL),分别用蒸馏水、30%乙醇、60%乙醇和95%乙醇洗脱,得到4种提取液,分别简称为蒸洗液、30%液、60%液、95%液。挥尽回收乙醇后,以蒸馏水转溶,调正体积(按比例折合含全方生药10g/mL),罐封冷藏备用 ......
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