温下方抑制肺癌A549细胞增殖及对PCNARbCyclinD1表达的影响(1)
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摘 要:目的:研究温下方对肺癌A549细胞增殖的抑制作用及对PCNA、Rb、CyclinD1表达的影响。方法:正常血清及不同浓度的温下方含药血清作用于A549细胞,MTT法检测含药血清对A549细胞的抑制作用,激光共聚焦显微镜观察PCNA、Rb、CyclinD1的表达。结果:温下方大、小剂量含药血清组作用24h抑制率为29.85%、25.90%,作用36h抑制率为42.02%、35.10%;PCNA表达降低至3.08±0.97、3.15±0.88,CyclinD1表达降低至2.65±0.83、2.70±0.79。结论:温下方可明显抑制肺腺癌细胞的增殖,明显降低PCNA、CyclinD1表达。
关键词:温下方;A549;PCNA;Rb;CyclinD1
中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)04-0721-03
Wenxia Formular Restrain the Multiplication of the A549
Cells and the Infection to the Expression of PCNA、Rb and CyclinD1
JI Xuming, OUYANG Bing, WANG Chunyan
(Shandong University of TCM, Jinan 250014, Shandong, China)
Abstract:Objectives: To investigate the restrain of Wenxia Formular to the multiplication of A549 cells and the influence to the expression of PCNA、Rb and CyclinD1. Methods: A549 cell lines were cultured with normal serum and different concentration of serum containing wenxia Formular. the MTT was used to exam the inhibition rate to A549 cells,he expression of PCNA、Rb and CyclinD1 in cells were evaluated by Laser Scanning Confocal Microscope.Results: After A549 cells 24h and 36h treated with wenxiafang big and small dose medicated serum,the rates of restrain were 29.85% and 25.90%,42.02% and 35.10%,The expression of PCNA and CyclinD1 significantly suppressed to 3.08±0.97,315±0.88 and 2.65±0.83,2.70±0.79(P<0.05).Conclusions: Wenxia Formular could significantly restrain the multiplication of A549 cell line, significantly suppress the expression of PCNA and CyclinD1.
Key words:Wenxia Formular; A549; PCNA;Rb; CyclinD1
[FQ(8。25,X-W]
收稿日期:2009-11-10
基金项目:国家教育部博士点基金资助项目(20050441007);中国博士后科学基金资助项目(20070410368)
作者简介:季旭明(1972-),女,内蒙古赤峰人,副教授,博士,主要从事中药复方效用机制及物质基础研究。
通讯作者:欧阳兵(1961-),男,山东日照人,教授,博士,主要从事中医文献的临床与实验研究。
2008年4月公布的第三次全国死因回顾调查表明,中国城乡居民的癌症死亡率在过去30年中增长了八成以上。肺癌稳居中国癌症死亡“排行榜”的首位,寻求有效的防治手段为当务之急。体内研究结果表明,温下方对小鼠Lewis肺癌有明显抑制作用[1],为进一步探讨其防治肺癌的作用,笔者研究温下方含药血清对肺腺癌细胞株A549的抑制作用及对增殖细胞核抗原(PCNA)、视网膜母细胞瘤肿瘤抑制蛋白(Rb)和周期素D1(CyclinD1)表达的影响,总结报道如下。
1材料仪器
1.1细胞株 肺腺癌细胞株 A549购于中国科学院生物物理所(北京),置于含10%小牛血清的 RPMI-1640培养液中,在 37℃、5%CO2条件下培养 ......
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