三七多糖含量测定方法比较(1)
摘要：目的 通过比较硫酸苯酚法和硫酸蒽酮法在测定三七多糖时的优劣性，建立更准确的三七多糖含量测定检测方法，为三七、三七剪口的多糖质量评价提供依据。方法 依次用80%乙醇和纯水提取三七药材，水提部分得到三七多糖，对所得多糖采用硫酸-苯酚法和硫酸-蒽酮法分别测量三七粗多糖的含量。结果 从精密度的角度来看，苯酚硫酸法较蒽酮硫酸法可靠(RSD=0.419<3.66)。从回收率的角度来看，使用苯酚硫酸法测定时，回收率更高(98.06>97.60)。结论 苯酚硫酸法为更准确测定三七粗多糖含量的检测方法，此外，苯酚硫酸法还具有操作更简便、安全、快捷等优点。
关键词：三七粗多糖;硫酸苯酚法;硫酸蒽酮法;含量测定
中图分类号：R285 文献标志码：A 文章编号：1007-2349(2020)08-0059-04
【Abstract】Objective： To establish a more accurate method for the determination and detection of notoginseng polysaccharide content by comparing the pros and cons of the sulfuric phenol method and the anthrone sulfate method in the determination of notoginseng polysaccharides and provide an evaluation basis for the quality of polysaccharides in notoginseng and notoginseng snip. Methods： The medicinal materials of Panax notoginseng were extracted with 80% ethanol and pure water in sequence， and the polysaccharides of Panax notoginseng were obtained by water extraction. The content of crude polysaccharides of Panax notoginseng was measured by the sulfuric acid-phenol method and the sulfuric acid-anthrone method. Results： From a precision point of view， the phenol sulfuric acid method was more reliable than the anthrone sulfuric acid method (RSD=0.419<3.66). From the perspective of recovery rate， the recovery rate is higher when the phenol sulfuric acid method was used for determination (98.06>97.60). Conclusion： The phenol-sulfuric acid method is a more accurate method to determine the content of the crude polysaccharides of Panax notoginseng. In addition， the phenol-sulfuric acid method is easier to operate， and safer and faster.
【Key words】crude polysaccharide of Panax notoginseng， phenol sulfate method， anthrone sulfate method， content determination
三七[Panax notoginseng(Burk.)F.H.Chen]，为五加科人参属多年生草本植物，主要药用部位为根部，产地主要为云南文山，广西等地[1]。三七为我国的传统名贵中药材，主要用于治疗外伤出血、咯血、吐血、便血、胸腹刺痛、跌打肿痛等[2]。三七皂苷为三七的主要活性成分，具有抗炎、抗肿瘤、提高免疫力、保護心血管、保肝保肾等药理作用[3]。三七多糖是三七另一个活性成分，在药材中含量为6%-10%左右[4]，无毒副作用[5]，药理研究表明三七多糖具有增强机体免疫力的作用，具有一定的开发价值。为此本文对三七中的多糖提取物进行了含量测定，比较了硫酸苯酚法和硫酸蒽酮法在测定三七多糖时的优劣性，为建立更准确的三七多糖含量测定方法和质量评价提供了参考依据。
1 材料、仪器和试剂
1.1 材料与试剂 材料：三七剪口皂苷提取后药渣干品所制得粗多糖(云科药业和云南三七科技有限公司技术中心提供);
试剂：无水乙醇、苯酚、浓硫酸、蒽酮(以上试剂均为分析纯);
标准曲线所用标准品：葡萄糖
1.2 仪器与设备 万能粉碎机;电子天平(梅特勒);电热恒温鼓风干燥箱(矛华DHG-9420);HH-4数显恒温水浴锅;型循环水真空泵;RE-2000A型旋转蒸发仪;超声波清洗器;紫外可见分光光度计(日历);双光束紫外可见分光光度计(上海驰唐实业有限公司);离心机(上海恒平);真空干燥箱(玛瑞特)。, http://www.100md.com(蔡群虎 刘石磊 袁敏惠 王芬 胡会泽 张芳盛 郭霞 刘晓丽 辛文锋)
关键词：三七粗多糖;硫酸苯酚法;硫酸蒽酮法;含量测定
中图分类号：R285 文献标志码：A 文章编号：1007-2349(2020)08-0059-04
【Abstract】Objective： To establish a more accurate method for the determination and detection of notoginseng polysaccharide content by comparing the pros and cons of the sulfuric phenol method and the anthrone sulfate method in the determination of notoginseng polysaccharides and provide an evaluation basis for the quality of polysaccharides in notoginseng and notoginseng snip. Methods： The medicinal materials of Panax notoginseng were extracted with 80% ethanol and pure water in sequence， and the polysaccharides of Panax notoginseng were obtained by water extraction. The content of crude polysaccharides of Panax notoginseng was measured by the sulfuric acid-phenol method and the sulfuric acid-anthrone method. Results： From a precision point of view， the phenol sulfuric acid method was more reliable than the anthrone sulfuric acid method (RSD=0.419<3.66). From the perspective of recovery rate， the recovery rate is higher when the phenol sulfuric acid method was used for determination (98.06>97.60). Conclusion： The phenol-sulfuric acid method is a more accurate method to determine the content of the crude polysaccharides of Panax notoginseng. In addition， the phenol-sulfuric acid method is easier to operate， and safer and faster.
【Key words】crude polysaccharide of Panax notoginseng， phenol sulfate method， anthrone sulfate method， content determination
三七[Panax notoginseng(Burk.)F.H.Chen]，为五加科人参属多年生草本植物，主要药用部位为根部，产地主要为云南文山，广西等地[1]。三七为我国的传统名贵中药材，主要用于治疗外伤出血、咯血、吐血、便血、胸腹刺痛、跌打肿痛等[2]。三七皂苷为三七的主要活性成分，具有抗炎、抗肿瘤、提高免疫力、保護心血管、保肝保肾等药理作用[3]。三七多糖是三七另一个活性成分，在药材中含量为6%-10%左右[4]，无毒副作用[5]，药理研究表明三七多糖具有增强机体免疫力的作用，具有一定的开发价值。为此本文对三七中的多糖提取物进行了含量测定，比较了硫酸苯酚法和硫酸蒽酮法在测定三七多糖时的优劣性，为建立更准确的三七多糖含量测定方法和质量评价提供了参考依据。
1 材料、仪器和试剂
1.1 材料与试剂 材料：三七剪口皂苷提取后药渣干品所制得粗多糖(云科药业和云南三七科技有限公司技术中心提供);
试剂：无水乙醇、苯酚、浓硫酸、蒽酮(以上试剂均为分析纯);
标准曲线所用标准品：葡萄糖
1.2 仪器与设备 万能粉碎机;电子天平(梅特勒);电热恒温鼓风干燥箱(矛华DHG-9420);HH-4数显恒温水浴锅;型循环水真空泵;RE-2000A型旋转蒸发仪;超声波清洗器;紫外可见分光光度计(日历);双光束紫外可见分光光度计(上海驰唐实业有限公司);离心机(上海恒平);真空干燥箱(玛瑞特)。, http://www.100md.com(蔡群虎 刘石磊 袁敏惠 王芬 胡会泽 张芳盛 郭霞 刘晓丽 辛文锋)