大株红景天的化学成分及体外抗肿瘤转移活性研究(1)
[摘要]采用硅胶柱色谱、凝胶柱色谱和制备HPLC色谱分离方法分离纯化大株红景天中的化学成分，采用有机波谱方法鉴定化合物结构，并以Transwell趋化实验方法测定化合物抗肿瘤转移作用。从大株红景天乙醇提取物中分离得到10个化合物，分别为丁香酸(1)，红景天苷(2)，酪醇(3)，scaphopetalone(4)，berchemol(5)，2，6-二甲氧基苯乙酮-4-O-β-D-吡喃葡萄糖苷(6)，柴胡红景天氰苷素A(7)，对苯乙烯基-O-β-D-木糖基-(1→6)-O-β-D-吡喃葡萄糖苷(8)，对苯乙烯基-4-O-β-D-呋喃芹菜糖基-(1→6)-O-β-D-吡喃葡萄糖苷(9)，丁子香基-4-O-β-呋喃芹菜糖基-(1→6)-O-β-吡喃葡萄糖苷(10)。化合物4～6和8～10均为首次从本属植物中分离得到，化合物7为首次从大株红景天中分离得到。抗肿瘤转移实验结果表明化合物2，6～8均有明确的抗肿瘤转移活性，其中化合物2和8的抗肿瘤转移活性较强。
[关键词]红景天属;大株红景天;化学成分;抗肿瘤转移活性
, 百拇医药
Anti-tumor metastatic constituents from Rhodiola wallichiana
CHAI Ya-qing1， ZHAO Guo-hua2， WANG Ren-jiu2， CAO Ming-guang2， WU Hai-bo2， TANG Sheng-an1， DUAN Hong-quan1，3，4*
(1. College of Pharmacy， Tianjin Medical University， Tianjin 300070， China;
2. Tonghua Yusheng Medicine Co.， Ltd. Tonghua 134000， China;
, 百拇医药
3. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics， Tianjin 300070， China;
4. Tianjin Research Center of Basic Medical Science， Tianjin 300070， China)
[Abstract]To study the anti-tumor metastatic constituents in Rhodiola wallichiana (HK) S H Fu var Cholaensis (Praeg) S H Fu， chemical constituents were isolated and purified by repeated column chromatography(silica gel， Toyopearl HW-40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the activities of the isolated compounds. Ten compounds(1-10) were isolated and their structures were identified by comparison of their spectral data with literature as follows： syringic acid(1)， salidroside(2)， tyrosol(3)， scaphopetalone(4)， berchemol(5)， 2，6-dimethoxyacetophenone(6)， rhobupcyanoside A(7)， miyaginin(8)， chavicol-4-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside(9)， eugenyol- O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside(10). Compounds 4-6 and 8-10， were isolated from this genus for the first time， while compound 7 was isolated from this plant for the first time. Compounds 2， 6-8 showed positive anti-tumor metastatic activities， and compounds 2 and 8 showed significant anti-tumor metastatic activities.
, 百拇医药
[Key words]Rhodiola; Rhodiola wallichiana; chemical constituents; anti-tumor metastatic activity
doi：10.4268/cjcmm20150218
大株红景天Rhodiolawallichiana(HK)SHFuvar.cholaensis(Praeg)SHFu为景天科红景天属植物，全名大株粗茎红景天[1]，分布于云南、青海及西藏等地。研究表明，红景天属植物具有抗氧，抗疲劳，抗紫外线，抗辐射，抗心肌缺血等功效[2-4]。大株红景天中含有红景天苷，酪醇，多糖，黄酮及挥发油类等多种生物活性成分[5-6]。, 百拇医药(柴雅晴 赵国华 王仁久 曹明光 吴海波 唐生安 段宏泉)
[关键词]红景天属;大株红景天;化学成分;抗肿瘤转移活性
, 百拇医药
Anti-tumor metastatic constituents from Rhodiola wallichiana
CHAI Ya-qing1， ZHAO Guo-hua2， WANG Ren-jiu2， CAO Ming-guang2， WU Hai-bo2， TANG Sheng-an1， DUAN Hong-quan1，3，4*
(1. College of Pharmacy， Tianjin Medical University， Tianjin 300070， China;
2. Tonghua Yusheng Medicine Co.， Ltd. Tonghua 134000， China;
, 百拇医药
3. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics， Tianjin 300070， China;
4. Tianjin Research Center of Basic Medical Science， Tianjin 300070， China)
[Abstract]To study the anti-tumor metastatic constituents in Rhodiola wallichiana (HK) S H Fu var Cholaensis (Praeg) S H Fu， chemical constituents were isolated and purified by repeated column chromatography(silica gel， Toyopearl HW-40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the activities of the isolated compounds. Ten compounds(1-10) were isolated and their structures were identified by comparison of their spectral data with literature as follows： syringic acid(1)， salidroside(2)， tyrosol(3)， scaphopetalone(4)， berchemol(5)， 2，6-dimethoxyacetophenone(6)， rhobupcyanoside A(7)， miyaginin(8)， chavicol-4-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside(9)， eugenyol- O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside(10). Compounds 4-6 and 8-10， were isolated from this genus for the first time， while compound 7 was isolated from this plant for the first time. Compounds 2， 6-8 showed positive anti-tumor metastatic activities， and compounds 2 and 8 showed significant anti-tumor metastatic activities.
, 百拇医药
[Key words]Rhodiola; Rhodiola wallichiana; chemical constituents; anti-tumor metastatic activity
doi：10.4268/cjcmm20150218
大株红景天Rhodiolawallichiana(HK)SHFuvar.cholaensis(Praeg)SHFu为景天科红景天属植物，全名大株粗茎红景天[1]，分布于云南、青海及西藏等地。研究表明，红景天属植物具有抗氧，抗疲劳，抗紫外线，抗辐射，抗心肌缺血等功效[2-4]。大株红景天中含有红景天苷，酪醇，多糖，黄酮及挥发油类等多种生物活性成分[5-6]。, 百拇医药(柴雅晴 赵国华 王仁久 曹明光 吴海波 唐生安 段宏泉)