超滤质谱技术筛选板蓝根中抗流感病毒的活性成分(4)
(1.College of Pharmacy， Chengdu University of Traditional Chinese Medicine， Chengdu 610075， China；
2. China Military Institute of Chinese Materia Medica， 302 Military Hospital， Beijing 100039， China；
3.College of Pharmacy， He′nan University of Traditional Chinese Medicine， Zhengzhou 450008， China)
[Abstract] In vitro neuraminidase inhibition assays and ultrafiltration liquid chromatography with diodearray detector coupled to time of flight mass spectrometer (UPLC-DAD-TOF-MS) were combined to screen bioactive compounds inhibiting neuraminidase from Isatidis Radix. By comparing the compounds from Isatidis Radix before and after ultrafiltration， we found that arginine， goitrin and adenosinea can bind with neuraminidase， and the binding degree of the three compounds were (36.23±1.12)%， (32.54±1.02)% and (9.38±0.47)%， respectively. The IC50of arginine and goitrin were (1.16±0.02)， (1.20±0.02) g·L-1， respectively. While the IC50of adenosinea was higher than 500 g·L-1. The results showed that arginine and goitrin might be the main compounds with antiviral activity of Isatidis Radix. This study may provide a useful method for the screening of bioactive compounds and quality control of Isatidis Radix.
[Key words] ultrafiltration mass spectrometry； Isatidis Radix； active compounds； inhibiting neuraminidase activity
doi：10.4268/cjcmm20140511
[责任编辑 马超一], 百拇医药(马丽娜 章从恩 鄢丹 谭曼容 李寒冰 张乐乐 熊吟 肖小河)
2. China Military Institute of Chinese Materia Medica， 302 Military Hospital， Beijing 100039， China；
3.College of Pharmacy， He′nan University of Traditional Chinese Medicine， Zhengzhou 450008， China)
[Abstract] In vitro neuraminidase inhibition assays and ultrafiltration liquid chromatography with diodearray detector coupled to time of flight mass spectrometer (UPLC-DAD-TOF-MS) were combined to screen bioactive compounds inhibiting neuraminidase from Isatidis Radix. By comparing the compounds from Isatidis Radix before and after ultrafiltration， we found that arginine， goitrin and adenosinea can bind with neuraminidase， and the binding degree of the three compounds were (36.23±1.12)%， (32.54±1.02)% and (9.38±0.47)%， respectively. The IC50of arginine and goitrin were (1.16±0.02)， (1.20±0.02) g·L-1， respectively. While the IC50of adenosinea was higher than 500 g·L-1. The results showed that arginine and goitrin might be the main compounds with antiviral activity of Isatidis Radix. This study may provide a useful method for the screening of bioactive compounds and quality control of Isatidis Radix.
[Key words] ultrafiltration mass spectrometry； Isatidis Radix； active compounds； inhibiting neuraminidase activity
doi：10.4268/cjcmm20140511
[责任编辑 马超一], 百拇医药(马丽娜 章从恩 鄢丹 谭曼容 李寒冰 张乐乐 熊吟 肖小河)