丹参脂溶性成分抑制体外血管新生及机制研究(3)
[11] 姚佳红，赵红卫，刘朝奇. VEGF及其受体研究新进展[J]. 现代医药卫生，2003，19(8)：973.
[12] 杨艳，葛荣明. 血管内皮生长因子 C的研究进展[J]. 同济大学学报：医学版，2001，22(3)：67.
Study on inhibitory effects and mechanism of lipophilic components in
Salvia miltiorrhiza on angiogenesis in vitro
FAN Xue-mei1，REN Gui-xiang2，LIANG Qiong-lin1*，WANG Yi-ming1，LUO Guo-an1
(1.Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology under Ministry of Education，Department of Chemistry，Tsinghua University，Beijing 100084，China；
2.School of Pharmacy，East China University of Science and Technology，Shanghai 200237，China)
[Abstract] In this study，the human umbilical vein endothelial cell model was used to study the regulating effect of lipophilic components in Salvia miltiorrhiza on angiogenesis，and explore its possible mechanism. The cell model was established to determine the effect of lipophilic components in S. miltiorrhiza on the proliferative activity and migration capacity of endothelial cells. Then the real-time fluorescence quantification PCR technology was applied to detect the changes in the gene expressions of angiogenesis-related cytokines VEGF-A，VEGF-C and MMP-9. The results showed that 5 mg·L-1 lipophilic components in S. miltiorrhiza could inhibit the proliferation and migration of endothelial cells，and reduce the expression of VEGF-A and MMP-9 genes. It indicated that lipophilic components in S. miltiorrhiza may inhibit the proliferation and migration of endothelial cells by inhibiting the expression of VEGF-A and MMP-9 genes，so as to show the inhibitory effect on angiogenesis.
[Key words] lipophilic components in Salvia miltiorrhiza； angiogenesis； human umbilical vein endothelial cells； Real-time fluorescence quantification PCR； vascular endothelial growth factor
doi：10.4268/cjcmm20140437, http://www.100md.com(范雪梅 任桂香 梁琼麟 王义明 罗国安)
[12] 杨艳，葛荣明. 血管内皮生长因子 C的研究进展[J]. 同济大学学报：医学版，2001，22(3)：67.
Study on inhibitory effects and mechanism of lipophilic components in
Salvia miltiorrhiza on angiogenesis in vitro
FAN Xue-mei1，REN Gui-xiang2，LIANG Qiong-lin1*，WANG Yi-ming1，LUO Guo-an1
(1.Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology under Ministry of Education，Department of Chemistry，Tsinghua University，Beijing 100084，China；
2.School of Pharmacy，East China University of Science and Technology，Shanghai 200237，China)
[Abstract] In this study，the human umbilical vein endothelial cell model was used to study the regulating effect of lipophilic components in Salvia miltiorrhiza on angiogenesis，and explore its possible mechanism. The cell model was established to determine the effect of lipophilic components in S. miltiorrhiza on the proliferative activity and migration capacity of endothelial cells. Then the real-time fluorescence quantification PCR technology was applied to detect the changes in the gene expressions of angiogenesis-related cytokines VEGF-A，VEGF-C and MMP-9. The results showed that 5 mg·L-1 lipophilic components in S. miltiorrhiza could inhibit the proliferation and migration of endothelial cells，and reduce the expression of VEGF-A and MMP-9 genes. It indicated that lipophilic components in S. miltiorrhiza may inhibit the proliferation and migration of endothelial cells by inhibiting the expression of VEGF-A and MMP-9 genes，so as to show the inhibitory effect on angiogenesis.
[Key words] lipophilic components in Salvia miltiorrhiza； angiogenesis； human umbilical vein endothelial cells； Real-time fluorescence quantification PCR； vascular endothelial growth factor
doi：10.4268/cjcmm20140437, http://www.100md.com(范雪梅 任桂香 梁琼麟 王义明 罗国安)