纤维素酶转化朝藿定B制备箭藿苷B的研究(4)
Study on preparation of sagittatoside B with epimedin B
converted from cellulase
XU Feng-juan 1，2， SUN E 2， ZHANG Zhen-hai 2， CUI Li 2 ， JIA Xiao-bin 1，2*
(1. School of Pharmacy， Jiangsu University， Zhenjiang 212013， China；
2. Key Laboratory of New Traditional Chinese Medicine Drug Delivery System under State Administration of
Traditional Chinese Medicine， Jiangsu Provincial Academy of Chinese Medicine， Nanjing 210028， China)
[Abstract]To prepare sagittatoside B with epimedin B Hydrolyzed from cellulase. With the conversion ratio as the index， the effects of pH value， temperature， reaction time， dosage of enzyme and concentration of substrates on the conversion ratio were detected. L9(34) orthogonal design was adopted to optimize the preparation process. Hydrolyzed products were identified by MS， 1H-NMR， and 13C-NMR. The results showed that the optimum reaction conditions for the enzymatic hydrolysis were that the temperature was 50 ℃， the reaction medium was pH 5.6 acetic acid-sodium acetate buffer solution， the concentration of substrates was 20 g·L-1， the mass ratio between enzyme and substrate was 3∶5， and the relative molecular mass of the reaction product was 646.23. NMR data proved that the product was sagittatoside B. The process is simple and reliable under mild reaction conditions， thus suitable for industrial production.
[Key words]sagittatoside B； epimedin B； cellulase； bioconversion
doi：10.4268/cjcmm20140214
[责任编辑马超一], http://www.100md.com(徐凤娟 孙娥 张振海 崔莉 贾晓斌)
converted from cellulase
XU Feng-juan 1，2， SUN E 2， ZHANG Zhen-hai 2， CUI Li 2 ， JIA Xiao-bin 1，2*
(1. School of Pharmacy， Jiangsu University， Zhenjiang 212013， China；
2. Key Laboratory of New Traditional Chinese Medicine Drug Delivery System under State Administration of
Traditional Chinese Medicine， Jiangsu Provincial Academy of Chinese Medicine， Nanjing 210028， China)
[Abstract]To prepare sagittatoside B with epimedin B Hydrolyzed from cellulase. With the conversion ratio as the index， the effects of pH value， temperature， reaction time， dosage of enzyme and concentration of substrates on the conversion ratio were detected. L9(34) orthogonal design was adopted to optimize the preparation process. Hydrolyzed products were identified by MS， 1H-NMR， and 13C-NMR. The results showed that the optimum reaction conditions for the enzymatic hydrolysis were that the temperature was 50 ℃， the reaction medium was pH 5.6 acetic acid-sodium acetate buffer solution， the concentration of substrates was 20 g·L-1， the mass ratio between enzyme and substrate was 3∶5， and the relative molecular mass of the reaction product was 646.23. NMR data proved that the product was sagittatoside B. The process is simple and reliable under mild reaction conditions， thus suitable for industrial production.
[Key words]sagittatoside B； epimedin B； cellulase； bioconversion
doi：10.4268/cjcmm20140214
[责任编辑马超一], http://www.100md.com(徐凤娟 孙娥 张振海 崔莉 贾晓斌)