丹参枯萎病及其病原菌的研究(3)
Chinese Academy of Agricultural Sciences， Beijing 100193， China；
3.College of Resources Science & Technology， Beijing Normal University， Beijing 100875， China)
[Abstract] Salvia miltiorrhiza is a highly valued traditional chinese medicine for the treatment of atherosclerosis-related disorders in china， such as cardiovascular and cerebrovascular diseases in China. The wilt disease is serious in the culture of S. miltiorrhiza. Wilt disease cause biomass of plant shoots and roots is lessened， active components are decreased. To solve these problems，we research the pathogen causing wilt disease of S. miltiorrhiza. The suspected pathogen is identified by morphology and etiological test. The identification was further confirmed by alignment the sequences of internal transcribed spacer( ITS) amplified by PCR. Our result show the wilt disease of S. miltiorrhiza mostly occurred in July and August， which is hot and wetter. The wilt disease rate of S. miltiorrhiza continuous cropping for one year in S.miltiorrhiz stubble is 10%， but the wilt disease rate of S. miltiorrhiza continuous cropping for three years in S.miltiorrhiz stubble is 60%-70%.The root rot of S.miltiorrhiz caused by the wilt disease， so the wilt disease was mistaken for the rot root in production. Morphological characteristics show the pathogen is Fusarium oxysporum. The sequence of ITS wes determined and found by BLAST shared 99% identity to that of F. oxysporum f.sp. cucumerinum. So it comes to the conclusion that the causing agent of wilt disease on S. miltiorrhiza belongs to F. oxysporum.
[Key words] Salvia miltiorrhiza； wilt disease； pathogen identification； Fusarium oxysporum
doi：10.4268/cjcmm20132309, http://www.100md.com(杨立 缪作清 杨光 邵爱娟 黄璐琦 申业 王雪 陈美兰)
3.College of Resources Science & Technology， Beijing Normal University， Beijing 100875， China)
[Abstract] Salvia miltiorrhiza is a highly valued traditional chinese medicine for the treatment of atherosclerosis-related disorders in china， such as cardiovascular and cerebrovascular diseases in China. The wilt disease is serious in the culture of S. miltiorrhiza. Wilt disease cause biomass of plant shoots and roots is lessened， active components are decreased. To solve these problems，we research the pathogen causing wilt disease of S. miltiorrhiza. The suspected pathogen is identified by morphology and etiological test. The identification was further confirmed by alignment the sequences of internal transcribed spacer( ITS) amplified by PCR. Our result show the wilt disease of S. miltiorrhiza mostly occurred in July and August， which is hot and wetter. The wilt disease rate of S. miltiorrhiza continuous cropping for one year in S.miltiorrhiz stubble is 10%， but the wilt disease rate of S. miltiorrhiza continuous cropping for three years in S.miltiorrhiz stubble is 60%-70%.The root rot of S.miltiorrhiz caused by the wilt disease， so the wilt disease was mistaken for the rot root in production. Morphological characteristics show the pathogen is Fusarium oxysporum. The sequence of ITS wes determined and found by BLAST shared 99% identity to that of F. oxysporum f.sp. cucumerinum. So it comes to the conclusion that the causing agent of wilt disease on S. miltiorrhiza belongs to F. oxysporum.
[Key words] Salvia miltiorrhiza； wilt disease； pathogen identification； Fusarium oxysporum
doi：10.4268/cjcmm20132309, http://www.100md.com(杨立 缪作清 杨光 邵爱娟 黄璐琦 申业 王雪 陈美兰)