过钒酸钠对照射后BET2细胞线粒体膜影响
BET-2细胞；电离辐射；线粒体跨膜电位(ΔΨm)；细胞凋亡；过钒酸钠,,BET-2细胞；电离辐射；线粒体跨膜电位(ΔΨm)；细胞凋亡；过钒酸钠,1材料与方法,2结果,3讨论,参考文献
摘要： 目的 研究酪氨酸磷酸酶抑制剂过钒酸钠(Pervanadate，Per)对电离辐射作用后细胞线粒体膜电位(ΔΨm)改变和细胞凋亡的影响。方法 应用流式细胞仪测定电离辐射作用后鼠源性髓系白血病细胞株BET-2细胞线粒体膜电位及其在过钒酸钠干预后的改变和细胞凋亡水平。结果 电离辐射早期可引起BET-2细胞线粒体膜电位降低，诱导细胞凋亡发生；过钒酸钠在一定程度上稳定细胞线粒体膜电位，减少细胞凋亡。结论 过钒酸钠可逆转电离辐射诱导的细胞线粒体膜电位的降低，减少造血细胞凋亡的发生。关键词： BET-2细胞；电离辐射；线粒体跨膜电位(ΔΨm)；细胞凋亡；过钒酸钠
Effects of pervanadate on MMP of BET-2 cells induced by ionizing irradiation
YANG Long,WANG Yi-ping,RONG Shu,et al.
Institute of Military Medical Research Center of Nanjing Military District (Nanjing 210002,China)
Abstract： Objective To study the effects of pervanadate(Per),the inhibitor of protein tyrosine phosphotases on the alterations of mitochondrial membrane potential(MMP)and the apoptosis of BET-2 cells induced by ionizing irradiation.Methods BET-2 cells were divided into Per-treated groups and non-Per groups and incubated after ionizing irradiation.The MMP and apoptosis were measured with FCM.Results After irradiation was given,the MMP of BET-2 cells was reduced in the early stage significantly,and the apoptosis appeared,and these changes had a dose-dependent pattern and a time-course fashion.Per potently enhanced the MMP of BET-2 cells and prevented irradiation-treated cells from apoptosis.Conclusion Per reversed the repression of mitochondrial membrane potential and suppress apoptosis induced by ionizing irradiation in the hematopoietic cells. ......
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