白花丹参根制剂对胃癌细胞增殖和凋亡的影响
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焦 鹏, 常 起, 杨明峰, 张 凤, 赵晓民, 夏作理
白花丹参根制剂;胃癌;凋亡焦鹏,常起,杨明峰,张凤,赵晓民,夏作理.白花丹参根制剂对胃癌细胞增殖和凋亡的影响. 世界华人消化杂志2007;15(8)820-823,焦鹏,杨明峰,张凤,赵晓民,常起,夏作理,焦鹏,通讯作者:,传真
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焦鹏, 杨明峰, 张凤, 赵晓民, 泰山医学院生命科学研究所 山东省泰安市 271000
常起, 泰山医学院附属医院人事科 山东省泰安市 271000
夏作理, 泰山医学院微循环重点实验室 山东省泰安市 271000
焦鹏, 2005年福建师范大学硕士, 主要从事细胞生物学的科研和教学工作.
通讯作者: 夏作理, 271000, 山东省泰安市迎胜东路2号, 泰山医学院微循环重点实验室. xiaminzhao@163.com
传真: 0538-6222600
收稿日期: 2006-12-06 接受日期: 2007-01-10
Effects of Salvia miltiorrhiza Bge.f.alba root preparation on the proliferation and apoptosis of gastric cancer cells
Peng Jiao, Qi Chang, Ming-Feng Yang, Feng Zhang, Xiao-Min Zhao, Zuo-Li Xia
Peng Jiao, Ming-Feng Yang, Feng Zhang, Xiao-Min Zhao, Institute of Life Sciences, Taishan Medical College, Tai’an 271000, Shandong Province, China
Qi Chang, Department of Personnel, the Affiliated Hospital of Taishan Medical College, Tai’an 271000, Shandong Province, China
Zuo-Li Xia, Key Laboratory of Microcirculatory, Taishan Medical College, Tai’an 271000, Shandong Province, China
Correspondence to: Zuo-Li Xia, Key Laboratory of Microcirculatory, Taishan Medical College, 2 Yingsheng East Road, Tai’an, 271000, Shandong Province, China. xiaminzhao@163.com
Received: 2006-12-06 Accepted: 2007-01-10
AbstractAIM: To investigate the effects of Salvia miltiorrhiza Bge.f.alba root preparation (SMRP) on the proliferation and apoptosis of human gastric cancer cell line BGC823 cultured in vitro.
METHODS:Human gastric cancer cells BGC823 cultured in vitro were treated with different concentrations of SMRP, and then the relative survival rate of the cells in each group was examined by MTT method. Flow cytometry was used to detect the apoptosis of BGC823 cells and laser scanning confocal microscopy (LSCM) was used to demonstrate cell morphology. Statistical analysis was performed and t test was used to compare the mean value between the different groups.
RESULTS:After treatment with different concentrations of SMRP, as measured by MTT assay, cell viability decreased in a dose-dependent manner (0.4 g/L: 91.7% ± 10.6%; 0.8 g/L: 66.8% ± 5.1%; 1 g/L: 57.5% ± 9.6%; 1.5 g/L: 32.6% ± 7.3%; 2 g/L: 29.4% ± 9.4%). BGC823 cells cultured with SMRP showed the typical morphologic characteristics of apoptosis. Flow cytometry showed that the apoptotic rates in the early and late stage after SMRP treatment were significantly higher than those in the controls (4.105% ± 2.393% vs 2.950% ± 1.575%, P < 0.05; 21.465% ± 6.474% vs 3.848% ± 2.264%, P < 0.05).
CONCLUSION: SMRP can inhibit the proliferation and induce the apoptosis of human gastric cancer cells.
Key Words: Salvia miltiorrhiza Bge.f.alba root preparation; Gastric cancer; Apoptosis; Proliferation; MTT assay; Flow cytometry
Jiao P, Chang Q, Yang MF, Zhang F, Zhao XM, Xia ZL. Effects of Salvia miltiorrhiza Bge.f.alba root preparation on the proliferation and apoptosis of gastric cancer cells. Shijie Huaren Xiaohua Zazhi 2007;15(8):820-823 ......
焦鹏, 杨明峰, 张凤, 赵晓民, 泰山医学院生命科学研究所 山东省泰安市 271000
常起, 泰山医学院附属医院人事科 山东省泰安市 271000
夏作理, 泰山医学院微循环重点实验室 山东省泰安市 271000
焦鹏, 2005年福建师范大学硕士, 主要从事细胞生物学的科研和教学工作.
通讯作者: 夏作理, 271000, 山东省泰安市迎胜东路2号, 泰山医学院微循环重点实验室. xiaminzhao@163.com
传真: 0538-6222600
收稿日期: 2006-12-06 接受日期: 2007-01-10
Effects of Salvia miltiorrhiza Bge.f.alba root preparation on the proliferation and apoptosis of gastric cancer cells
Peng Jiao, Qi Chang, Ming-Feng Yang, Feng Zhang, Xiao-Min Zhao, Zuo-Li Xia
Peng Jiao, Ming-Feng Yang, Feng Zhang, Xiao-Min Zhao, Institute of Life Sciences, Taishan Medical College, Tai’an 271000, Shandong Province, China
Qi Chang, Department of Personnel, the Affiliated Hospital of Taishan Medical College, Tai’an 271000, Shandong Province, China
Zuo-Li Xia, Key Laboratory of Microcirculatory, Taishan Medical College, Tai’an 271000, Shandong Province, China
Correspondence to: Zuo-Li Xia, Key Laboratory of Microcirculatory, Taishan Medical College, 2 Yingsheng East Road, Tai’an, 271000, Shandong Province, China. xiaminzhao@163.com
Received: 2006-12-06 Accepted: 2007-01-10
AbstractAIM: To investigate the effects of Salvia miltiorrhiza Bge.f.alba root preparation (SMRP) on the proliferation and apoptosis of human gastric cancer cell line BGC823 cultured in vitro.
METHODS:Human gastric cancer cells BGC823 cultured in vitro were treated with different concentrations of SMRP, and then the relative survival rate of the cells in each group was examined by MTT method. Flow cytometry was used to detect the apoptosis of BGC823 cells and laser scanning confocal microscopy (LSCM) was used to demonstrate cell morphology. Statistical analysis was performed and t test was used to compare the mean value between the different groups.
RESULTS:After treatment with different concentrations of SMRP, as measured by MTT assay, cell viability decreased in a dose-dependent manner (0.4 g/L: 91.7% ± 10.6%; 0.8 g/L: 66.8% ± 5.1%; 1 g/L: 57.5% ± 9.6%; 1.5 g/L: 32.6% ± 7.3%; 2 g/L: 29.4% ± 9.4%). BGC823 cells cultured with SMRP showed the typical morphologic characteristics of apoptosis. Flow cytometry showed that the apoptotic rates in the early and late stage after SMRP treatment were significantly higher than those in the controls (4.105% ± 2.393% vs 2.950% ± 1.575%, P < 0.05; 21.465% ± 6.474% vs 3.848% ± 2.264%, P < 0.05).
CONCLUSION: SMRP can inhibit the proliferation and induce the apoptosis of human gastric cancer cells.
Key Words: Salvia miltiorrhiza Bge.f.alba root preparation; Gastric cancer; Apoptosis; Proliferation; MTT assay; Flow cytometry
Jiao P, Chang Q, Yang MF, Zhang F, Zhao XM, Xia ZL. Effects of Salvia miltiorrhiza Bge.f.alba root preparation on the proliferation and apoptosis of gastric cancer cells. Shijie Huaren Xiaohua Zazhi 2007;15(8):820-823 ......
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