(Jiangsu Provincial Center for Research and Development of Marine Drugs, Nanjing University of Traditional Chinese Medicine, Jiangsu Nanjing 210029, China )

    Abstract:ObjectiveTo establish a RP-HPLC method for determination of the content of Paeoniflorin in Zishenligan Granule. MethodsThe analysis was carried out by using a Kromasil C18 analytical column with methanol and 0.05mol·L-1 potassium dihydrogen phosphate solution (32:68, v/v) as mobile phase. The UV detection wavelength was 230nm and the flow rate was 1ml·min-1. ResultsUnder the chromatographic conditions used, there was no interference found in the position of Paeoniflorin peak. Paeoniflorin had fine linear responses (r=0.999 9) in the concentration range of 24.28～121.40 μg·ml-1, the average recovery of Paeoniflorin in Zishenligan Granule was 98.32% with the relative standard deviation of 1.34% (n=9). ConclusionThe method employed in this analysis is convenient with good repetition and can be used as a reliable tool for quality control in the production of Zishenligan Granule.

    Key words:Zishenligan Granule； Paeoniflorin； Reversed-Phase High Performance Liquid Chromatography； Quantitative Analysis ......