21味寒水石散；_没食子酸；_含量测定；

高效液相色谱法测定21味寒水石散中没食子酸的含量

http://www.100md.com 《时珍国医国药》 2006年第11期

21味寒水石散；,没食子酸；,含量测定；,高效液相色谱法,,21味寒水石散；,没食子酸；,含量测定；,高效液相色谱法,1仪器与试药,2方法与结果,3讨论,参考文献：

     摘要：目的建立21味寒水石散中没食子酸的含量测定方法。方法采用高效液相色谱(HPLC)法测定21味寒水石散中没食子酸的含量，用十八烷基硅烷键合硅胶为填充剂，甲醇水(5∶95)为流动相，检测波长为274 nm。结果线性范围0.020 6～0.247 2 μg，平均回收率为99.1%，RSD=1.3% (n=9)。结论该方法简便、准确，提供了21味寒水石散中没食子酸的质量控制方法。

    关键词：21味寒水石散；没食子酸；含量测定；高效液相色谱法

    Determination of Gallic Acid in 21component Hanshuishi Powder by HPLC

    LIANG Yongxin，LIN Pengcheng

    (Chemistry Department of Qinghai Nationalities College, Xi'ning 810007,China)

    Abstract：ObjectiveTo establish a quantitative method to determine the content of gallic acid in 21component Hanshuishi Powder. MethodsThe content of gallic acid in 21component Hanshuishi powder was determined by HPLC. The separation was performed on Kromasil C18 column with a mobile phase of methanol-water (5∶95) and detected at wavelength of 274 nm.ResultsThe linearity of the method was good at range of 0.020 6～0.247 2 μg and the average recovery of gallic acid was 99.1%,RSD was 1.3%.ConclusionThe method is convenient, accurate, and can be used for the determination of gallic acid in 21component Hanshuishi Powder. ......

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