吴茱萸；_高效液相色谱；_指纹图谱____

吴茱萸高效液相色谱指纹图谱的研究

http://www.100md.com 《时珍国医国药》 2006年第11期

吴茱萸；,高效液相色谱；,指纹图谱,,吴茱萸；,高效液相色谱；,指纹图谱,1仪器与试药,2方法与结果,3讨论,参考文献：

     摘要：目的研究吴茱萸药材高效液相色谱 (HPLC)指纹图谱的测定方法。方法Alltima C18柱(4.6 mm×150 mm,5 μm)；流动相∶乙腈∶甲醇∶水(2.0%四氢呋喃+0.4%醋酸)=45∶3∶52；检测波长：225 nm；流速：1.0 ml/min；柱温：25℃。结果HPLC指纹图谱共有8个色谱峰 (RSD≤3%)，符合有关规定。结论所建立的 HPLC指纹图谱为吴茱萸的质量控制提供了更全面的信息,并为吴茱萸及含吴茱萸药材的中成药指纹图谱研究奠定了基础。

    关键词：吴茱萸；高效液相色谱；指纹图谱

    The Study of HPLC Fingerprint of Fructus Evodiae

    MO Shanlie, LI Zhan,OU Ying, WEI Song,SI Xiuling

    (Guangxi Traditional Chinese Medical University,Nanning 530001，China)

    Abstract：ObjectiveTo study the HPLC fingerprint of Fructus Evodiae. MethodsChromatographic conditions included Alltima C18 column and the mobile phase composed of a mixture of acetonitrile carbinol water. Detection wavelength at 225nm. The mobile phase flow rate was 1.0 ml/min. ResultsThe HPLC fingerprint consisted of 8 peaks(RSD≤3%)，and reached to some relevant provision. ConclusionThe method can provide more information for the quality control of Fructus Evodiae, and it may be practically valuable for the quality control of sample for Fructus Evodiae and it's preparation. ......

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