[摘要] 目的 研究血管内皮细胞生长因子(VEGF)在胃癌患者血清及肿瘤组织中的表达情况，探讨其临床意义。方法 应用ELISA法对60例非肿瘤疾病手术患者和60例胃癌患者术前、术后血清VEGF水平进行定量测定，并应用免疫组化法测定肿瘤组织的VEGF。结果 胃癌患者术前血清VEGF高于对照组(P>0.05)。两组术后较术前升高，肿瘤组术后较术前升高(P<0.05)。肿瘤组术前血清VEGF水平、肿瘤组织中的VEGF与浆膜受累、淋巴结转移及临床病理分期有关。肿瘤组术后随访30～36个月中出现复发转移和死亡的患者，术前VEGF表达较其他患者升高(P<0.05)。结论 血清VEGF水平及肿瘤组织中的VEGF与胃癌的浸润、转移有关，检测血清VEGF及肿瘤组织中的VEGF对胃癌浸润、转移程度和预后的判断有重要意义。

    [关键词] 胃肿瘤；微转移；血管内皮生长因子

    Expression and significance of VEGF in serum and tumor tissue in gastric cancer

    ZHOU Hai-yin,LI Jian,QIAO Shi-ming,et al.Department of General Surgery,The Central Hospital of Yangpu District,Shanghai 200090,China

    [Abstract] Objective To evaluate the clinical significance of vascular endothelial growth factor (VEGF) expression in serum and tumor tissue in gastric cancer patients.Methods The pre-operational and post-operational levels of serum VEGF were measured by ELISA kit in 60 cases of gastric cancer and 60 cases of non-carcinoma as the control group,the expression of tumor tissue VEGF was measured by immunohistochemical assay.Results The pre-operational level of serum VEGF in gastric cancer was higher than that in the control group(P>0.05).The post-operational level of serum VEGF was increased in both groups,especially in the tumor group(P<0.05).The expression of VEGF in serum and tumor tissue was positively correlated with tumor invasion depth,lymph-node metastasis and clinical pathological stage.A follow up of 30～36 months reveals the pre-operational level of serum VEGF of tumor recurrence and death in patients was higher than the others in the tumor group(P<0.05).Conclusion The expression of serum VEGF and tumor tissue VEGF is closely correlated with invasion and metastasis of gastric cancer.Serum VEGF and tumor tissue VEGF assay may be used to pre-operatively predict invasion and metastasis and used to evaluate prognosis. ......